Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. 0. The Catalina. SpectraMax® Microplate Readers and SoftMax® Pro Software. (Shiga, Japan) and kept for 30 min in the dark. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. edu Contact Phone: 216-368-8962. Details. gp70 blocking. Lactate Dehydrogenase (LDH) Release. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. Novak, Nathan J. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. A. Details. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. Wavelength Range. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . The SpectraMax® iD3 Multi Mode Microplate . Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. using SpectraMax i3 (Molecular devices, San Jose, USA). Number of flashes. Molecular Devices. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. mums. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SpectraMax Multi-Mode Microplate Readers. 6. 5. , Sunnyvale, CA). Microplate Spectrophotometer Operator’s Manual. 5. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. Acceptor: 665nm. albicans pre. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. 5. Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). 5 mL sample or standard solution was well mixed with 0. The reaction was stopped with 1 M hydrochloric acid after 15 min. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Call 1-800-745-2710. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. using SpectraMax i3 (Molecular devices, San Jose, USA). The concentration of TNFα in the experimental. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. 6) and 0. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. , Oxon, UK). Features showcased by this model include. Description 1. 001/min at 425 nm. Donor: 620 nm. 1 mL of culture media with 10% FBS. Wavelength range: 340-850 nm. ): 8. SpectraMax Microplate Readers. All strains were tested three times and the results averaged. 1% N-1-naphtylethylenediamine dihydrochloride, 2. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). Ehrlich assay for quantification of 5-ALA A 200 μL 5-ALA sample was mixed with 200 μL sodium acetate (pH 4. Warranty. Six wells were assayed for each set of conditions and stadard deviations. To. Photometric Range: 0. Percent Transmission Absorbance Competitor 0. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. Homeostatic model assessment for insulin. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. FACS analysis. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. Upgradeable modules are also available including western blot, cell imaging, and fast. 0% and ± 0. , Sunnyvale, CA, USA) at 595 nm. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. California Lightworks uses only the highest quality LEDs from Osram. SpectraMax 190 reads 96-well and strip-well plates. 5), L-ascorbic acid (25. After adding sample, the mixture was incubated for 30 min at 37°C, with one unit of enzymatic activity being defined as an increase in absorbance of 0. 003 OD Stray light ≤ 0. Absorbance was recorded at 540 nm using SpectraMAX 340 reader. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. 0, MSD) or Spectramax 340 (Molecular Devices). Details. The ABS reader ranges from. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 0. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. Subsequently, the mixture was heated at 100 °C for 10. Oxidative stress induction by H 2 O. GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The VersaMax and SpectraMax 190 read 96-well microplates. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. , Sunnyvale, CA, USA) at 595 nm. 1 mg mL −1 CDDP loaded A-CHAM, B-CHAM, C-CHAM or D-CHAM. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The VersaMax and SpectraMax 190 read 96-well microplates. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. 2 Chemical Reagents 1. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). 190-1000 nm. Each SpectraMax® 340 PC 384 Microplate Reader has an. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Anti-proteolytic activity. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. 0200-0729. control. 312 gain calibration failed. SPECTRAmax® 190. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. Donor: 616/10 nm. Gerritsma,B. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. 5. In vivo anti-malarial assay in mice. Call 1-800-745-2710. 0200-6117. 5. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Stat Fax 4700 Microstrip Reader. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 2 software was used to measure and calculate of the bioactivity activity studies. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. Values were determined according to a standard curve (log/log curve-fit). Manager: Yinghua Chen, PhD Contact Email: yinghua. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. 340 nm / 70 nm. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. The plate was covered and incubated for one hour at room temperature. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Also for: Spectramax 190, Spectramax 340pc384, Versamax. SpectraMax ® Plus 384 optics. Acceptor filter: 665/10 nm. plates can be used in the SPECTRAmax PLUS 384. 4. Create custom fluorescence assays without having to purchase expensive filter sets. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. 0, 20 mmol L –1cal-cium chloride). 97), and each plate contained at least six positive and six negative controls. Details. 2. ). The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Molecular Devices SpectraMax Gemini XS Microplate Reader. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. AoSMCs were seeded into 6-well culture plates. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Each experiment was performed in duplicate and repeated at. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. control. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The first time you replace an OEM lamp you must use the N-34-MLA. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). 5 mL of 1 M acetate buffer (pH 4. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). Water – distilled water or equivalent. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. Molecular Devices SpectraMax M5e Microplate Reader $26,500. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 190–850 nm <± 1. SpectraMax Multi-Mode Microplate Readers. 304 signal level saturation. When necessary, samples were. Stat Fax 3200 Microplate Reader. 4) was followed according to Vanacker et al. Unit has several areas of damaged plastic. Immunofluorescence and Aggregation Analysis. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Microplate Spectrophotometers. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Each experiment was performed in duplicate and repeated at. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. Wells were washed three times. Values were determined according to a standard curve (log/log curve-fit). Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. They are for reference only. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 570nm on the indicated days (Cell Proliferation Kit I; Roche). Donor filter: 620 nm. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. Cell proliferation was measured using the MTT method. 30. 30. used in the SPECTRAmax PLUS. Call 1-800-745-2710. The SPECTRAmax 340PC can mea- TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. 30. 315 can't find zero order. Molecular Devices SpectraMax M5e Microplate Reader. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). Stat Fax 4700 Microstrip Reader. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Our absorbance plate readers feature our PathCheck Sensor technology and. control or celatrol-treated cells. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. Examples: “Press carefully to engage,” “Do not press down. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Description. 00 USD. StakMax Microplate Handling Stacker. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. using SpectraMax i3 (Molecular devices, San Jose, USA). TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. Heme was preincubated for 5 min with A when added before the metals. ) at 490 nm. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. 306 plate air cal fail,low light. The ratio of red to green fluorescence intensity was calculated. Molecular Devices Spectramax 340PC instructions. Please see photos. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). g. Service parts for these models are not being produced and are no longer in stock. One plate carrier adapter is provided with the instrument. Alves, Amelia K. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Statistical analysis. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Donor filter: 616/10 nm. ) to the upper chamber, followed by measurement with a spectrophotometer (SpectraMax 340, Molecular Devices LLC, Sunnyvale, CA, USA) at 610 nm of media in the lower chamber after one hour . After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. 5), L-ascorbic acid (25. #N207) LAL Reagent Water (Lonza cat. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. , Fremont, CA, USA). The microtiter plate was then incubated in the dark for 2 h at 37°C. 2. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). VersaMax reads 96-well and strip-well plates. 190–850 nm <± 1. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Integration delay. Both readers feature an 8-channel read head for fast reads and. SpectraMax Injector Cartridge with SmartInject™ Technology. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. If depot service is the best option for you we will assist you with logistics to help you keep. Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. 2 - 3. SpectraMax Gemini EM & FLEXstation (bottom read) 1. The SPECTRAmax 340PC can measure. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. 04. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Nitric Oxide Assay. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. PerkinElmer. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). The initial rate of hydrolysis was determined using the early linear region of. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. , Sunnyvale, Calif. SoftMax Pro. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. TGFα release was calculated as described in the Results. 3. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). 5 g/L-EDTA 0. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). 4. Analysis of ALA by Ehrlich’s Reagent Assay. SpectraMax 340 from Molecular Devices). IMAP ® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. Details. Quick View. This reader only allows high performance HTRF measurement when assays are run in. Awareness Technology Inc. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. . Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. Other technologies such as imaging. 30 µs. Subsequent replacements require only the lamp (N-34-LB) be replaced. Cross sections of mouse skin was prepared and stained as previously described . Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. D. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. TDP-43 stress granular cells were treated with 70% ice-cold. SpectraMax Multi-Mode Microplate Readers. Details. Customer Transformation Hub. The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. SPECTRAmax® 190. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. Buffer (100 mmol L –1acetate buffer, pH 5. 340 nm / 80 nm.